Leptospirosis dan Pengembangan Metode Deteksi Leptospirosis Pada Sapi
Abstract
ABSTRAK
Leptospirosis merupakan penyakit zoonosis yang disebabkan oleh Leptospira sp. Kejadian leptospirosis di Indonesia masih belum menjadi perhatian utama pemangku kebijakan. Hal ini disebabkan karena leptospirosis umumnya tidak menimbulkan gejala klinis yang signifikan pada ternak. Namun, masih diduga kuat bahwa leptospirosis menimbulkan penurunan produksi dan kerugian ekonomi. Selain itu potensi zoonosis yang ditimbulkan harus menjadi perhatian utama. Metode deteksi leptospirosis saat ini masih terbatas pada microscopic agglutination test (MAT). Metode uji ini masih ditemukan banyak kekurangan sehingga seiring waktu dilakukan pengembangan metode lain, seperti histopatologi, enzyme-linked immunosorbent assays (ELISA) maupun reverse-trancriptase polymerase chain reaction (RT-PCR). Sehingga pemahaman tentang metode pengujian yang akurat untuk leptospirosis harus ditingkatkan. Hal ini terkait dengan teknik diagnostika dalam pencegahan, pengendalian, dan pemberantasan leptospirosis pada ternak di Indonesia.
Kata kunci: histopatologi, leptospirosis, MAT, RT-PCR, zoonosis.
ABSTRACT
Leptospirosis is a zoonosis disease that caused by Leptospira sp. The evidence of leptospirosis in Indonesia still not becoming the major of concern of the government in policies developing. It is caused by the leptospirosis clinical signs among the livestock generate asymptomatically. However, leptospirosis is suspected causes the decrease of production and economic losses. In addition, the potency of zoonosis must be came a major concern. The detection method of leptospirosis is still using microscopic agglutination test (MAT). This method is found have several limitations compared to enzyme —linked immunosorbent assays (ELISA) and reverse-transcriptase polymerase chain reaction (RT-PCR). The understanding regarding the accurate detection methods for leptospirosis should be increased. It is related to the diagnostic method on prevention, control, and eradication of leptospirosis among the livestock in Indonesia.
Keywords: histopathology, leptospirosis, MAT, RT-PCR, zoonosis.
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DAFTAR PUSTAKA
Li, S., Wang, D., Zhang, C., Wei, X., Tian, K., Li, X., Nie, Y., Liu, Y., Yao, G., Zhou, J., Tang, G., Jiang, X., & Yan, J. (2013). Source tracking of human leptospirosis: serotyping and genotyping of Leptospira isolated from rodents in the epidemic area of Guizhou province, China. BMC microbiology, 13, 75.https://doi.org/10.1186/1471-2180-13- 75.
Schneider, M. C., Jancloes, M., Buss, D. F., Aldighieri, S., Bertherat, E., Najera, P., Galan, D. I., Durski, K., & Espinal, M. A. (2013). Leptospirosis: a silent epidemic disease. International journal of environmental research and public health, 10(12), 7229—7234. https://doi.org/10.3390/ijerph10127229
Levett P. N. (2001). Leptospirosis. Clinical microbiology reviews, 14(2), 296—326. https://doi.org/10.1128/CMR.14.2.296- 326.2001.
Schafbauer, T., Dreyfus, A., Hogan, B., Rakotozandrindrainy, R., Poppert, S., & Straubinger, R. K. (2019). Seroprevalence of Leptospira spp. Infection in Cattle from Central and Northern Madagascar. International journal of environmental research and public health, 16(11), 2014. https://doi.org/10.3390/ijerph16112014
Patra, K. P., Choudhury, B., Matthias, M. M., Baga, S., Bandyopadhya, K., & Vinetz, J. M. (2015). Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species. BMC microbiology, 15, 244. https://doi.org/10.1186/s12866-015- 0581-7.
Benacer, D., Mohd Zain, S. N., Amran, F., Galloway, R. L., & Thong, K. L. (2013). Isolation and molecular characterization of Leptospira interrogans and Leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia. The American journal of tropical medicine and hygiene, 88(4), 704—709. https://doi.org/10.4269/ajtmh.12-0662.
Lilenbaum, W., & Martins, G. (2014). Leptospirosis in cattle: a challenging scenario for the understanding of the epidemiology. Transboundary and emerging diseases, 61 Suppl 1, 63—68. https://doi.org/10.1111/tbed.12233.
Almeida, D. S., Paz, L. N., de Oliveira, D. S., Silva, D. N., Ristow, P., Hamond, C., Costa, F., Portela, R. W., Estrela-Lima, A., & Pinna, M. H. (2019). Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. PloS one, 14(5), e0217391. https://doi.org/10.1371/journal.pone.02 17391.
Evangelista, K. V., & Coburn, J. (2010). Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responses. Future microbiology, 5(9), 1413—1425. https://doi.org/10.2217/fmb.10.102.
Kingscote B. F. (1985). Leptospirosis in livestock. The Canadian veterinary journal = La revue veterinaire canadienne, 26(8), 235—236.
Niloofa, R., Fernando, N., de Silva, N. L., Karunanayake, L., Wickramasinghe, H., Dikmadugoda, N., Premawansa, G., Wickramasinghe, R., de Silva, H. J., Premawansa, S., Rajapakse, S., & Handunnetti, S. (2015). Diagnosis of Leptospirosis: Comparison between Microscopic Agglutination Test, IgM-ELISA and IgM Rapid Immunochromatography Test. PloS one, 10(6), e0129236. https://doi.org/10.1371/journal.pone.01 29236.
Yitzhaki, S., Barnea, A., Keysary, A., & Zahavy, E. (2004). New approach for serological testing for leptospirosis by using detection of leptospira agglutination by flow cytometry light scatter analysis. Journal of clinical microbiology, 42(4), 1680-1685. https://doi.org/10.1128/JCM.42.4.1680-1685.2004.
Rosa, M. I., Reis, M., Simon, C., Dondossola, E., Alexandre, M. C., Colonetti, T., & Meller, F. O. (2017). IgM ELISA for leptospirosis diagnosis: asystematic review and meta- analysis. Ciencia & saude coletiva, 22(12), 4001—4012. https://doi.org/10.1590/1413- 812320172212.14112016.
Sakamoto, S., Putalun, W., Vimolmangkang, S., Phoolcharoen, W., Shoyama, Y., Tanaka, H., & Morimoto, S. (2018). Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites. Journal of natural medicines, 72 (1), 32—42. https://doi.org/10.1007/s11418-017- 1144-z.
Stoddard R. A. (2013). Detection of pathogenic Leptospira spp. through real-time PCR (qPCR) targeting the LipL32 gene. Methods in molecular biology (Clifton, N.J.), 943, 257—266. https://doi.org/10.1007/978-1-60327- 353-4_17.
Zippelius, A., Kufer, P., Honold, G., Köllermann, M. W., Oberneder, R., Schlimok, G., Riethmüller, G., & Pantel, K. (1997). Limitations of reverse-transcriptase polymerase chain reaction analyses for detection of micrometastatic epithelial cancer cells in bone marrow. Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 15(7), 2701—2708. https://doi.org/10.1200/JCO.1997.15.7. 2701.
Waggoner, J. J., & Pinsky, B. A. (2016). Molecular diagnostics for human leptospirosis. Current opinion in infectious diseases, 29(5), 440—445. https://doi.org/10.1097/QCO.00000000 00000295.
Doungchawee, G., Sutdan, D., Niwatayakul, K., Inwisai, T., Sitthipunya, A., Boonsathorn, N., Sakulterdkiat, T., Sirawaraporn, W., & Thongboonkerd, V. (2017). Development and evaluation of an immunochromatographic assay to detect serum anti-leptospiral lipopolysaccharide IgM in acute leptospirosis. Scientific reports, 7(1), 2309. https://doi.org/10.1038/s41598- 017-02654-8.
Cheow, L. F., Ko, S. H., Kim, S. J., Kang, K. H., & Han, J. (2010). Increasing the sensitivity of enzyme- linked immunosorbent assay using multiplexed electrokinetic concentrator. Analytical chemistry, 82(8), 3383—3388. https://doi.org/10.1021/ac9024335.
Naf'an, M. K., Kurniasih, K., Untari, T., & Prakoso, Y. A. (2020). Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry. Veterinary world, 13(8), 1719—1724. https://doi.org/10.14202/vetworld.20201719-1724.
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